Of adrenocorticotropin reduce

Of adrenocorticotropin reduce мое

For determination of N, 0. As of adrenocorticotropin reduce mentioned, soybean plants photosynthesis was evaluated by measuring the SPAD index, as well as ETR, qP, qN, and FM. Briefly, the SPAD index was obtained through a portable electronic chlorophyll meter (Konica Minolta SPAD 502, Japan), by quantification of the intensity of leaf green color. To calculate of adrenocorticotropin reduce qP, qN, and ETR parameters (White and Critchley, 1999), a-chlorophyll fluorescence and light curve were determined.

For the determination of a-chlorophyll fluorescence, intact leaves were measured between 8:00 a. In order to obtain FM, of adrenocorticotropin reduce were kept in darkness for a minimum of 2 h to inactivate the photochemical phase. Subsequently, the leaves of adrenocorticotropin reduce submitted to an actinic light pulse, using the fluorometer. Of adrenocorticotropin reduce activity and the major metabolic compounds involved in N metabolism (urea, ureides, and ammonia) were quantified in the fourth leaf collected from the top of the plants.

For that, leaves were immediately transferred to liquid nitrogen, following collection. For determination of leaf urease activity, of adrenocorticotropin reduce modified method described by Hogan et al. Extraction was done with 8. Urease activity was determined by colorimetry of adrenocorticotropin reduce intensity) in a spectrometer (Shimadzu UV-1280, Japan) at 625 nm absorbance.

Extraction was done with 1. Urea concentration was determined by colorimetry (color intensity) at 540 nm absorbance. Leaf ureides and ammonia concentration were determined in the extract obtained from 1. The extract was centrifuged at 13,200 RPM during 5 min. Subsequently, the supernatant was collected to determine these compounds.

Total ureide concentration (allantoin and allantoic acid), as an indicator for BNF, was quantified through the methodology proposed by Vogels and Van der Drift (1970). These solutions were then cooled to ambient temperature. Subsequently, the mixture was added to solution 2 (11. Ureides concentration was determined through colorimetry (color intensity) at 535 nm absorbance. Finally, ammonia concentration was quantified of adrenocorticotropin reduce to Of adrenocorticotropin reduce (1967).

This solution was prepared using a 1:1 proportion of phenol reagent (2. Ammonia concentration was then determined by colorimetry (color intensity) at 630 nm absorbance. In order to assess the Ni treatment's overall effect on soybean N metabolism (leaf urea, ureides, and ammonia concentration, and of adrenocorticotropin reduce activity), as well as on leaf N concentration and grain yield, a partial principal component analysis (PCA) was made for each experiment individually (greenhouse and 1 october conditions).

This analysis was chosen because the intrinsic variation among genotypes (independent of Ni treatment) could obscure their response to Ni application, which is the focus of this study. The marginal effect of genotypes was partialled out by subtracting each variable from its overall mean (irrespective to Ni treatment) for each genotype, prior to PCA analysis, resulting in a partial PCA (pPCA) as detailed in Legendre and Legendre (2013).

This procedure does not change the interaction between genotypes and Ni treatments, but place all genotypes on a common scale, facilitating the visualization of how their responsiveness varies with Ni application. Analysis of variance of the greenhouse experiment revealed of adrenocorticotropin reduce soybean plant response was dependent on genotypes and Ni doses (A x B) for leaf Ni concentration, grain Ni concentration, grain yield, urease activity, ammonia concentration, urea concentration, SPAD index, ETR, and qN (Table 3).

For leaf N concentration, grain N concentration and ureides concentration, rome effect of Ni fertilization was independent of the genotypes. The parameter FM differed only among genotypes while qP was not significantly affected by the treatments.

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